Myosin Heavy Chain (MHC) isoforms can be separated using acrylamide gels made with glycerol (Talmadge & Roy (1993), Blough et al (1996) and Reiser and Kline (1998). In collaboration with scientists at the University of Michigan Medical Center in Ann Arbor, the PCL has separated MHC isoforms in rat Soleus and Extensor digitalis longus muscle according to the procedure outlined by Reiser (1998). This procedure is now being applied to experimental samples provided by Dr. Melissa Melvin, the Department of Surgery.
Sol EDL
Dr. Melvin is growing functional muscle in vivo in Dr. David Brown's Tissue Engineering laboratory at the University of Michigan Medical Center. The lab has succeeded in developing functional tissue-engineered skeletal muscle in rats, with its own blood vessel and nerve supply. The current goal of the research is to identify the mechanisms involved in innervating the new muscles with various nerves, as a means to maximize force and endurance for specific needs. Engineered muscles would be widely beneficial to patients with congenital or acquired muscle loss and/or paralysis.
If you are interested in this procedure, please contact the PCL.
References:
Talmadge, R.J. and R.R. Roy (1993) Electrophoretic separation of rat skeletal muscle myosin heavy-chain isoforms. J. Appl. Physiol. 75, 2337-2340.
Blough, E.R, Rennie, E.R., Zhang, F. And P.J. Reiser (1996) Enhanced electrophoretic separation and resolution of myosin heavy chanis in mammalian skeletal muscles. Anal. Biochem. 233, 31-35.
Reiser, P.J and W.O. Kline (1998) Electrophoretic separation and quantiatin of cardiac myosin heavy chain isoforms in eight mammalian species. J. Appl. Physiol. 43, 1048-1053.
Myosin Heavy Chain (MHC) isoforms can be separated using acrylamide gels made with glycerol (Talmadge & Roy (1993), Blough et al (1996) and Reiser and Kline (1998). In collaboration with scientists at the University of Michigan Medical Center in Ann Arbor, the PCL has separated MHC isoforms in rat Soleus and Extensor digitalis longus muscle according to the procedure outlined by Reiser (1998). This procedure is now being applied to experimental samples provided by Dr. Melissa Melvin, the Department of Surgery.
Sol EDL
Dr. Melvin is growing functional muscle in vivo in Dr. David Brown's Tissue Engineering laboratory at the University of Michigan Medical Center. The lab has succeeded in developing functional tissue-engineered skeletal muscle in rats, with its own blood vessel and nerve supply. The current goal of the research is to identify the mechanisms involved in innervating the new muscles with various nerves, as a means to maximize force and endurance for specific needs. Engineered muscles would be widely beneficial to patients with congenital or acquired muscle loss and/or paralysis.
If you are interested in this procedure, please contact the PCL.
References:
Talmadge, R.J. and R.R. Roy (1993) Electrophoretic separation of rat skeletal muscle myosin heavy-chain isoforms. J. Appl. Physiol. 75, 2337-2340.
Blough, E.R, Rennie, E.R., Zhang, F. And P.J. Reiser (1996) Enhanced electrophoretic separation and resolution of myosin heavy chanis in mammalian skeletal muscles. Anal. Biochem. 233, 31-35.
Reiser, P.J and W.O. Kline (1998) Electrophoretic separation and quantiatin of cardiac myosin heavy chain isoforms in eight mammalian species. J. Appl. Physiol. 43, 1048-1053.