PROTEIN MICROSEQUENCING

Edman, P. (1950) Method for Determination of the Amino Acid Sequence in Peptides. Acta Chem. Scand., 4, 283-293.

Niall, H.D. (1973) Automated Edman degradation: the protein sequenator. Methods Enzymol. 27: 942-1010.

Matsudaira, P. (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidinedifluoride membranes. J. Biol. Chem. 262: 10035-10038.

SDS POLYACRYLAMIDE GEL ELECTROPHORESIS

Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227, 680-685.

Schagger, H. and Von Jagow, G. (1987). Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379.

2-D GEL ELECTROPHORESIS

Tube Gel - Based 2-D Gel Electrophoresis

Immobilized pH Gradient - Based 2-D Gel Electrophoresis

Bjellqvist, B., Ek, K., Righetti, P.G., Gianazza,E., Gőrg, A., Westermeirer, R., Postel, W. (1982) Isoelectric focusing in immobilized pH gradients: principle, methodology and some applications. J. Biochem. Biophys. Methods 6, 317-339.

Gőrg, A., Postel, W., Günther, S., Weser, J. (1985) Improved horizontal two-dimensional electrophoresis with hybrid isoelectric focusing in immobilized pH gradients in the first dimension and laying-on transfer to the second dimension. Electrophoresis 6, 599-604.

Gőrg, A., Postel, W., Günther, S. (1988) The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 9, 531-546.

ELECTROBLOTTING

Mozdzanowsky, J., Hembach, P. and Speicher, D.W. (1992). High yield electroblotting onto polyvinylidinedifluoride membranes from polyacrylamide gels. Electrophoresis 13: 59-64.

IN GEL DIGESTION PROCEDURES

Shevchenko, A., Tomas, H., Havlis, J., Olsen, J.V. and Mann, M. (2006). In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocols 1: 2856-2860.

SILVER STAINING

Blum, H., Beier, H. and Gross, H.J. (1987). Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93-99.

SILVER DESTAINING

Gharahdaghi, F., Weinberg, C.R., Meagher, D.A., Imai, B.S. and Mische, S.M. (1999) Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: a method for the removal of silver ions to enhance sensitivity. Electrophoresis 20: 601-605.

ACCELERATED PROTEIN DIGESTION

Russel, W.K., Park, Z.Y. and Russell, D.H. (2001) Proteolysis in mixed organic-aqueous solvent systems: applications for peptide mass mapping using mass spectrometry. Ana. Chem. 73: 2682-2685.

Park, Z-Y. and Russell, D.H. (2000) Thermal denaturation: a useful technique in peptide mass mapping. Anal. Chem. 72: 2667-2670.

DIFFERENCE GEL ELECTROPHORESIS (DIGE)

Unlu, M., Morgan, M.E. and Minden, J.S. (1997) Difference gel electrophoresis: a single gel method for detecting changes in protein extracts. Electrophoresis 18: 2071-2077.

Karp, N.A. and Lilley, K.S. (2005) Maximising sensitivity for detecting changes in protein expression: experimental design using minimal CyDyes. Proteomics. (12):3105-15.

Karp, N.A., McCormick, P.S., Russell, M.R. and Lilley, K.S. (2007) Experimental and statistical considerations to avoid false conclusions in proteomics studies using differential in-gel electrophoresis. Mol Cell Proteomics. 6(8):1354-64.

Fu, C., Hu, J., Liu, T., Ago, T., Sadoshima, J. and Li, H. Quantitative analysis of redox-sensitive proteome with DIGE and iCAT. (2008) J Proteome Res. 7(9): 3789–3802.

Karp, N.A. and Lilley, K.S. (2009) Investigating sample pooling strategies for DIGE experiments to address biological variability. Proteomics. 9(2):388-97.