Larry Dangott Ph.D Director 440A Bio/Bio 2128 Texas A&M University College Station, TX 77843-2128 Phone: (979) 845-2965 Fax: (979) 845-8015

Larry received his Bachelor of Arts degree in Biology from California State University, San José and his Masters of Arts and Ph.D. in Biology from the University of Oregon where he was trained in comparative biochemistry of non-vertebrate respiratory proteins. Larry's recent research interests center on gamete interactions and signaling and include the structure and function of receptors on sperm involved in the activation of sperm motility by factors from the female reproductive system. In addition, he continues his long-standing interest in the structure and function of extracellular hemoglobins and hemocyanins. He specializes in the identification and purification of integral membrane receptors and the use of photoaffinity labels and chemical cross linking agents, gel electrophoresis, HPLC and protein sequencing of hydrophobic peptides.

Terwilliger, R.C., Terwilliger, N.V., Schabtach, E., and Dangott, L.J. Erythrocruorins of Euzonus mucronata (Treadwell): Evidence for a dimeric annelid extracellular hemoglobin. Comp. Biochem. Physiol. 57A (1977) 143-149.

Dangott, L.J. and Terwilliger, R.C. Structural studies of a branchiopod crustacean (Lepidurus bilobatus) extracellular hemoglobin: Evidence for oxygen binding domains. Biochem. Biophys. Acta 579 (1979) 452-461.

Holliday, C.W., Mykles, D.L., Terwilliger, R.C., and Dangott, L.J. Fluid secretion by the midgut caeca of the crab Cancer magister. Comp. Biochem. Physiol. 67A (1980) 259-261.

Dangott, L.J. and Terwilliger, R.C. Subunit structure of Daphnia pulex hemoglobin. Comp. Biochem. Physiol. 67B (1980) 301-306.

Dangott, L.J. and Terwilliger, R.C. Arthropod extracellular hemoglobins: Structural and functional properties. Comp. Biochem.. Physiol. 70B (1981) 549-447.

Dangott, L.J. and Cunningham, L.W. Residual a2-macroglobulin in fetal calf serum and properties of its complex with thrombin. Biochem. Biophys. Res. Comm. 107 (1982) 1243-1251.

Dangott, L.J., Puett, D., and Cunningham., L.W. Conformational changes induced in human a2-macroglobulin protease and nucleophilic modification. Biochemistry 22 (1983) 3647-3653.

Dangott, L.J., Puett, D., and Cunningham, L.W. Changes in a2-macroglobulin conformation with trypsin and methylamine. Annals of the New York Academy of Science 421 (1983) 158-159.

Dangott, L.J., Puett, D., Garbers, D.L., and Melner, M.H. Tyrosine protein kinase activity in purified Leydig cells. Biochem. Biophys. Res. Comm. 116 (1983) 400-407.

Dangott, L.J., Puett, D., Garbers, D.L., Melner, M.H. (1983). Tyrosine protein kinase activity in purified Leydig cells. Biochem. Biophys. Res. Comm., 116, 400-407.

Dangott, L.J., Garbers, D.L. (1984). Identification and partial characterization of the receptor for speract. J. Biol. Chem., 259, 13712-13716.

Melner, M.R., Puett, D., Garbers, D.L., Dangott, L.J. (1985). Testicular tyrosine specific kinase activity: High levels in purified Leydig cells. Eighth Testis Workshop on Hormonal Control of Testicular Function, Bethesda, MD, Ann. N.Y. Acad. Sci., 431, 632-635.

Dangott, L.J., Puett, D., Melner, M.H. (1986). Characterization of protein tyrosine kinase activity in murine Leydig tumor cells. Biochem. Biophys. Acta., 886, 187-194.

Dangott, L.J., Terwilliger, R.C. (1986). The role of extracellular hemoglobins in the oxygen consumption of the burrowing Polychaete, Euzonus mucronata (Treadwell). J. Exp. Mar. Biol., 97, 193-204.

Dangott, L.J., Garbers, D.L. (1986). Peptides associated with eggs which regulate spermatozoa. News in Physiological Sciences, 1, 128-130.

Garbers, D.L., Noland, T.D., Dangott, L.J., Ramarao, C.S., Bentley, J.K. (1986). The interaction of egg peptides with spermatozoa. In: Molecular and Cellular Aspects of Reproduction (Dindsa, D.S., Bahl, D.P., eds.), Plenum Publishing Corporation, pp. 145-164.

Garbers, D.L., Bentley, J.K., Dangott, L.J., Ramarao, C.S., Shimomura, H., Suzuki, N., Thorpe, D. (1986). Peptides associated with eggs: Mechanisms of interaction with spermatozoa. In: Advances in Experimental Medicine and Biology, Vol. 201, The Molecular and Cellular Biology of Fertilization (Hedrick, J.L., ed.), Plenum Press, New York, pp. 315-357.

Shimomura, H., Dangott, L.J., Garbers, D.L. (1986). Covalent coupling of a resact analogue to guanylate cyclase. J. Biol. Chem., 261, 15778-15782.

Garbers, D.L., Shimomura, H., Ramarao, C.S., Dangott, L.J., Bentley, J.K. (1986). The mechanism of action of egg peptides on spermatozoa. In: New Horizons in Sperm Cell Research (Mohri, H., ed.), Tokyo, Japan, Sci. Soc. Press, pp. 159-168.

Dangott, L.J., Garbers, D.L. (1988). Further characterization of a speract receptor on sea urchin spermatozoa. Ann. N.Y. Acad. Sci., 531, 274-285.

Singh, S., Lowe, D.G., Thorpe, D.S., Rodriguez, H., Kuang, W.-J., Dangott, L.J., Chinkers, M., Goeddel, D.V., Garbers, D.L. (1988). Membrane guanylate cyclase is a cell-surface receptor with homology to protein kinases. Nature, 334, 708-712.

Garbers, D.L., Lowe, D.G., Dangott, L.J., Chinkers, M., Thorpe, D.S., Bentley, J.K., Ramarao, C.S., Goeddel, D.V., Garbers, D.L. (1988). The membrane form of guanylate cyclase. In: The Molecular Biology of Signal Transduction, 53rd Cold Spring Harbor Symposium in Quantitative Biology, pp. 993-1003.

Dangott, L.J., Jordan, J.E., Bellet, R.A., Garbers, D.L. (1989). Cloning of the mRNA for the protein that crosslinks to the egg peptide speract. Proc. Natl. Acad. Sci. USA, 86, 2128-2132.

Cardullo, R.A., Herrick, S., Dangott, L.J. (1994). Speract receptors are localized on sea urchin sperm flagella using a fluorescent peptide analog. Dev. Biol., 162, 600-607.

Dangott, L.J. (1995). Peptide receptors in sea urchin sperm flagella. In: Methods in Cell Biology, Vol. 47, Cilia and Flagella (Dentler, W., Witman, G., eds.), Academic Press, New York, pp. 437-444.

Wilson, C.J., Husain, S.S., Stimson, E.R., Dangott, L.J., Miller, K.W., Maggio, J.E. (1997). p-(Hydroxybenzoyl)phenylalanine: A photoreactive amino acid analog amenable to radioiodination for elucidation of peptide-protein interaction. Biochemistry, 36, 4542-4557.

Blanton, M.P., Dangott, L.J., Raja, S.K., Lala, Cohen, J.B. (1998). Probing the structure of the nicotinic acetylcholine receptor ion channel with the uncharged photoactivatable compound [3H]diazofluorene. J. Biol. Chem., 273, 8659-8668.

Blanton, M.P., Yu Xie, Dangott, L.J., Cohen, J.B. (1999). The steroid promegestone is a noncompetitive antagonist of the Torpedo nicotinic acetylcholine receptor which interacts with the lipid-protein interface. Mol. Pharm., 55, 269-278.

Terwilliger, N.B., Dangott, L.J., Ryan, M. (1999). Cryptocyanin, crustacean molting protein: Evolutionary link with arthropod hemocyanins and insect hexamerins. Proc. Natl. Acad. Sci. USA, 96, 2013-2018.

Gadda, G., Dangott, L.J., Johnson, Jr., W.H., Whitman, C.P., Fitzpatrick, P.F. (1999). Characterization of 2-Oxo-3-pentynoate as an active-site-directed inactivator of flavoprotein oxidases: Identification of active-site peptides in tryptophan-2-monooxygenase. Biochemistry, 38, 5822-5828.

Noland, B.W., Dangott, L.J., Baldwin, T.O. (1999) Folding, stability, and physical properties of the a subunit of bacterial luciferase. Biochemistry, 38, 16136-16145.

Gadda, G., Banerjee, A., Dangott, L.J and Fitzpatrick, P.F. (2000) Identification of a cysteine residue in the active site of nitroalkane oxidase by modification with N-ethylmaleimide. J. Biol. Chem., 275, 31891-31895.

Consoli, F.L., Conti, E., Dangott, L.J. and Vinson, S.B. (2001) In vitro culture of the teratocytes of Trissolcus basalis (Hymentoptera, Scelionidae) and their requirements for host-derived components. Biological Control, 22, 176-184.

Holderman M.T., Miller K.P., Dangott L.J., Ramos K.S. (2002) Identification of albumin precursor protein, Phi AP3, and alpha-smooth muscle actin as novel components of redox sensing machinery in vascular smooth muscle cells. Mol Pharmacol. May;61(5):1174-83.

Apuy, J., Park, Z.-Y., Swartz, P.D., Dangott, L.J., Russell, D.H. and Baldwin, T.O. (2001) Pusled-alkylation mass spectrometry for the study of protein folding and dynamics: development and applications to the study of a folding/unfolding intermediate of bacterial luciferase. Biochemistry 40(50):15153-63.

Jordan, M.C. Harrington, J.R., Cohen, N.D. Tsolis,R.M., Dangott, L.J., Weinberg, E.D. and Martens, R.J. (2003) Effects of iron modulation on growth and viability of Rhodocoddus equi and expression of virulence-associated protein A. Am. J. Vet. Res. 64: 1337-1346.

Wang, X.X., Dangott, L.J. and Pfenninger, K.H. (2003) The heterogeneous growth cone glycoprotein gp93 is identical to the signal regulatory protein SIRPα/SHPS-1/BIT. Journal of Neurochemistry, 86(1), 55-60.

Carnes, M.L., Martens, R.L., Cohen, ND., Tsolis, R.M., Dangott, L.J., Weinberg, E.D. and Harrington, J.R. (2003) Rhodococcus equi: effects of iron modulation on growth and expression of a 23kDa virulence associated immunogenic protein. Am. J. Vet. Research, 64:1337-1346.

Woodard, S.L., Mayor, J.M., Bailey, M.R., Barker, D.K., Love,R.T., Lane, J.R., Delaney, D.E., McComas-Wagner, J.M., Mallubhotla, H.D., Hood, E.E., Dangott, L.J., Tichy, S.E. and Howard, J.A.(2003). Maize-derived bovine trypsin: the first large-scale, commercial protein product from transgenic plants. Biotechnol Appl Biochem. 38: 123-130.

Chiara, D.C., Dangott, L.J., Eckenhoff, R.G. and Cohen, J.B. (2003) Identification of nicotinic acetylcholine receptor amino acids photolabeled by the volatile anesthetic halothane. Biochemistry 25: 13457-67.

Samuel, S., Twizere, J-C, and Bernstein, L.R., (2005). YB-1 represses AP1-dependent gene transactivation and interacts with anAP-1 DNA sequence. Biochem. J. 388; 921-928.

Han, B.-K., Bogonolnaya, L.M., Totten, J.M., Blank. H.M., Dangott, L.J. and Polymenis, M. (2005) Bem1p, a scaffold signaling protein, mediates cyclin-dependent control of vacuolar homeostasis in Saccharomyces cerevisiae. Genes and Development 19: 2606 – 2618.

Djonivic, S., Pozo, M.J., Dangott, L.J., Howell, C.R and Kenerley, C.M. (2006) A small-cycteine rich protein secreted by the biocontrol fungus Trichoderma virens is an elicitor of plant defense responses. Mol. Plant-Microbe Interactions 19: 838-853.

Sacharidou, A. Halbig, K., Cifuentes-Rojas, C. Nova-Ocampo, M., Dangott, L.J. and Cruz-Reyes, J. (2006) RNA editing complex interactions with a site for full-round U deletion in procyclic and bloodstream Trypanosoma burcei. RNA 12: 1219-1228.

Wang, J., Li, D., Dangott, L.J. and Wu, G. (2006) Proteomics and its role in nutrition science. J. Nutrition 136: 1759-1762.

Wang J., Chen, L., Li, D., Yin, Y., Wang, X., Li, P., Dangott, L.J., Hu, W., and Wu, G. (2008) Intrauterine Growth Restriction Affects the Proteomes of the Small Intestine, Liver and Skeletal Muscle in Newborn Pigs. J. Nutrition; 138: 60-66.

Lee, S.C., Schmidtke, S.N., Dangott, L.J., and Shaw, B.D. (2008) Aspergillus nidulans ArfB is linked to endocytosis and polarized growth. Eukaryot. Cell. 7: 1278-1288.